築山 智之

Tomoyuki TSUKIYAMA
特任准教授(京都大学ASHBi 霊長類ゲノム工学開発コア)
経歴
平成14年3月 洛南高等学校卒業
平成18年3月 京都大学農学部卒業
平成21年4月 日本学術振興会特別研究員(DC2)
平成23年3月 京都大学大学院農学研究科修了
平成23年4月 理化学研究所 発生再生科学総合研究センター(理研CDB)研究員(丹羽 仁史 研究室)
平成26年4月 滋賀医科大学 動物生命科学研究センター 特任助教
平成26年11月 滋賀医科大学 動物生命科学研究センター 助教
平成31年4月 滋賀医科大学 動物生命科学研究センター (京都大学ASHBi 滋賀医大サテライト 霊長類ゲノム工学開発コア) 特任准教授

 

研究テーマ

新規培養条件によるナイーブES・iPS細胞の樹立とCRISPR/Cas9システムによる遺伝子改変サルの作製

メッセージ

個人ホームページ
再生医療が描く未来 -iPS細胞とES細胞-

講義資料
2019年度「発生学」講義
第2回「生殖細胞の発生、排卵と着床」こちら
第3回「二層性胚盤、三層性胚盤」こちら
第4回「胚子期後半、胎盤と胎膜」こちら

 

論文一覧
2019
  • Kobayashi K. #, Tsukiyama T. *,#, Nakaya M., Kageyama S., Tomita K., Murai R., Yoshida T., Narita M., Kawauchi A., and Ema M. * Generation of an OCT3/4 reporter Cynomolgus monkey ES cell line using CRISPR/Cas9. Stem Cell Research. in press (2019) (* Corresponding author, # Equal contribution)
  • Seita Y., Tsukiyama T., Azami T., Kobayashi K., Iwatani C., Tsuchiya H., Nakaya M., Tanabe H., Hitoshi S., Miyoshi H., Nakamura S., Kawauchi A., and Ema M. Comprehensive evaluation of ubiquitous promoters suitable for the generation of transgenic cynomolgus monkeys. Biology of Reproduction. in press (2019)
  • Watanabe C., Matsushita J., Azami T., Tsukiyama-Fujii S., Tsukiyama T., Mizuno S., Takahashi S., and Ema M. Generating Vegfr3 reporter transgenic mouse expressing membrane-tagged Venus for visualization of VEGFR3 expression in vascular and lymphatic endothelial cells. PLoS ONE. 14(1): e0210060. (2019)
2017
  • Honda A., Choijookhuu N., Izu H., Kawano Y., Inokuchi M., Honsho K., Lee AR., Nabekura H., Ohta H., Tsukiyama T., Ohinata Y., Kuroiwa A., Hishikawa Y., Saitou M., Jogahara T., and Koshimoto C. Flexible adaptation of male germ cells from female iPSCs of endangered Tokudaia osimensis. Science advances. 3(5): e1602179. (2017)
2016
  • Kawaguchi T., Cho D., Hayashi M., Tsukiyama T., Kimura K., Matsuyama S., Minami N., Yamada M. and Imai H. Derivation of Induced Trophoblast Cell Lines in Cattle by Doxycycline-Inducible piggyBac Vectors. PLoS One. 11(12):e0167550. (2016)
  • Khoa le TP, Azami T, Tsukiyama T, Matsushita J, Tsukiyama-Fujii S, Takahashi S, Ema M. Visualization of the Epiblast and Visceral Endodermal Cells Using Fgf5-P2A-Venus BAC Transgenic Mice and Epiblast Stem Cells. PLoS One. 11(7):e0159246. (2016)
  • Seita Y, Tsukiyama T, Iwatani C, Tsuchiya H, Matsushita J, Azami T, Okahara J, Nakamura S, Hayashi Y, Hitoshi S, Itoh Y, Imamura T, Nishimura M, Tooyama I, Miyoshi H, Saitou M, Ogasawara K, Sasaki E, Ema M. Generation of transgenic cynomolgus monkeys that express green fluorescent protein throughout the whole body. Sci Rep. 6:24868. (2016)
2015
  • Kawaguchi T., Tsukiyama T., Kimura K., Matsuyama S., Minami N., Yamada M. and Imai H. Generation of naive bovine induced pluripotent stem cells using piggyBac transposition of doxycycline-inducible transcription factors. PLoS ONE 10:e0135403. (2015)
  • Suzuki S., Tsukiyama T., Kaneko T., Imai H. and Minami N. A hyperactive piggyBac transposon system is an easy-to-implement method for introducing foreign genes into mouse preimplantation embryos. The Journal of Reproduction and Development, 61:241-244. (2015)
2014
  • Ohinata Y. and Tsukiyama T. Establishment of trophoblast stem cells under defined culture conditions in mice. PLoS ONE 9:e107308. (2014)
  • Tsukiyama T. and Ohinata Y. A Modified EpiSC Culture Condition Containing a GSK3 Inhibitor Can Support Germline-Competent Pluripotency in Mice. PLoS ONE 9:e95329. (2014)
  • Tsukiyama T., Kato-Itoh M., Nakauchi H. and Ohinata Y. A Comprehensive System for Generation and Evaluation of Induced Pluripotent Stem Cells Using piggyBac Transposition. PLoS ONE 9:e92973. (2014)
2011
  • Tsukiyama T., Asano R., Kawaguchi T., Kim N., Yamada M., Minami N., Ohinata Y. and Imai H. Simple and efficient method for generation of induced pluripotent stem cells using piggyBac transposition of doxycycline-inducible factors and an EOS reporter system. Genes to Cells 16:815-825. (2011)
  • Miyamoto K., Nagai K., Kitamura N., Nishikawa T., Ikegami H., Binh NT., Tsukamoto S., Matsumoto M., Tsukiyama T., Minami N., Yamada M., Ariga H., Miyake M., Kawarasaki T., Matsumoto K. and Imai H. Identification and characterization of an oocyte factor required for development of porcine nuclear transfer embryos. Proceedings of the National Academy of Sciences of the United States of America 8:7040-7045. (2011)
2009
  • Miyamoto K., Tsukiyama T., Yang Y., Li N., Minami N., Yamada M. and Imai H.  Cell-Free Extracts from Mammalian Oocytes Partially Induce Nuclear Reprogramming in Somatic Cells. Biology of Reproduction 80:935-943. (2009)
2008
  • Miyamoto K., Yamashita T., Tsukiyama T., Kitamura N., Minami N., Yamada M., Imai H. Reversible Membrane Permeabilization of Mammalian Cells Treated with Digitonin and Its Use for Inducing Nuclear Reprogramming by Xenopus Egg Extracts. Cloning and Stem Cells. 10:535-542. (2008)
2006
  • Watanabe T., Takeda A., Tsukiyama T., Mise K., Okuno T., Sasaki H., Minami N. and Imai H. Identification and Characterization of Two Novel Classes of Small RNAs in the Mouse Germline: Retrotransposon-Derived siRNAs in Oocytes and Germline Small RNAs in Testes. Genes & Development 20:1732-1743. (2006)